期刊论文

Analysis of bacterial diversity in Guangxi non-salt fermentation bamboo shoots based on Illumina MiSeq sequencing technology

中文

中国酿造

0254-5071

2019

38

7

85-90

10.11882/j.issn.0254-5071.2019.07.017

长沙市科技局重点项目(Kq1801152) 国家自然科学基金面上项目(31571819).

本校为第一机构

以10年老酸水泡制酸笋样品(B SFA F)、发酵6个月块状酸笋(M B SF)和丝状酸笋样品(FB SF)为研究对象,应用Illum ina M iSeq高通量测序技术并通过生物信息统计与分析,探索广西无盐发酵酸笋样品中细菌菌群结构及多样性.结果表明:3组酸笋样品共得到148656个有效的序列数(Tags),聚类后总共得到1116个分类操作单元(O TU s).根据物种注释结果,在3组酸笋样品中共注释到12个门、23个纲、37个目、98个科和190个属.在门分类水平上,3组样品中厚壁菌门(Firm icutes)占绝对优势,其次是变形杆菌门(Proteobacteria)和放线菌门(A ctinobacteria);在属分类水平上,乳杆菌属(Lactobacillus)、乳球菌属(Lactococcus)和...

Using the 10-year-old mixture sample (BSFAF),6-month-old massive (MBSF) and filamentous (FBSF) fermented sour bamboo shoot as research objects,the structure and diversity of bacterial flora in the sample of non-salt fermentation bamboo shoot in Guangxi were explored using Illumina MiSeq high-throughput sequencing technology and biometric information. A total of 148 656 valid Tags were obtained from 3 groups of samples,and a total of 1 116 operational taxonomic units (OTUs) were obtained after clustering. According to the results of species annotation,12 phyla,23 classes,37 orders,98 families a...