期刊论文

中文

中国酿造

0254-5071

2019

38

1

80-83

10.11882/j.issn.0254-5071.2019.01.016

湖南省科技计划项目(2017GK4032) 国家自然科学基金面上项目(31571811) 中国博士后科学基金项目(2017M622579).

本校为第一机构

该研究从自然发酵剁辣椒中分离筛选一株高产胞外多糖的乳酸菌,经形态观察、生理生化试验及分子生物学对其进行鉴定,并探索菌株产胞外多糖的最佳碳源和最佳培养时间.结果 表明,筛选并鉴定得到一株高产胞外多糖的植物乳杆菌(Lactobacillus plantarum) Y-20.菌株Y-20产胞外多糖的最佳碳源为麦芽糖,且在MRS培养基中培养16h时,OD600mm值达到最大(3.62),胞外多糖产量达到最高,为242.95 mg/L.

In this study,a high-yield extracellular polysaccharide lactic acid bacterium was isolated and screened from natural fermented chopped pepper,and identified by morphological observation,physiological and biochemical tests and molecular biology.The optimal carbon source and culture time for the strain producing extracellular polysaccharide were explored.The results showed that Lactobacillus plantarum Y-20 with high-yield extracellular polysaccharide was screened and identified.The optimal carbon source for the strain Y-20 was maltose,and the OD600 nm value reached maximum(3.62)and the yield of ...