期刊论文

中文

中国酿造

0254-5071

2019

38

1

75-79

10.11882/j.issn.0254-5071.2019.01.015

中国博士后科学基金项目(2017M622579) 湖南省科技计划项目(2017GK4032) 湖南省教育厅科学研究项目(15K058) 长沙市科技计划项目(kq1706025).

本校为第一机构

通过比色法测定酶活,比较5株植物乳杆菌(Lactobacillus plantarum)产β-葡萄糖苷酶的特性,对胞内β-葡萄糖苷酶最佳反应pH和温度进行了研究,考察了氯化钠及乙醇对β-葡萄糖苷酶活性的影响.结果 表明,5株植物乳杆菌都可以产生胞内p-葡萄糖苷酶,其中比酶活最高的为菌株W-4 (14.168 U/g),其次是菌株Y-12(13.015 U/g),之后分别是菌株Y-20 (11.359 U/g)、菌株1.3919 (7.029 U/g)及菌株Y-22 (4.630 U/g);植物乳杆菌W-4和1.3919还可以产生胞外酶;菌株W-4的胞内β-葡萄糖苷酶的最适反应pH为4.0～4.5,菌株Y-12的最适pH为4.5,菌株Y-20和1.3919的最适pH为5.5,菌株Y-22的最适pH为5.0;菌株W-4、Y-20、Y-22及1...

The characteristics ofβ-glycosidase produced by 5 strains of Lactobacillus plantarum were compared by colorimetric method.The optimum reaction temperature and pH of intracellularβ-glycosidase were studied,and the effects of sodium chloride and ethanol onβ-glycosidase activity were also investigated.The results showed that all strains could produce intracellularβ-glycosidase,among them,the strain W-4 had the highest enzyme activity(14.168 U/g),followed by Y-12(13.015 U/g),Y-20(11.359 U/g),1.3919(7.029 U/g)and Y-22(4.630 U/g),and the strain W...