期刊论文

中文

中国酿造

0254-5071

2020

39

3

63-67

10.11882/j.issn.0254-5071.2020.03.013

现代农业产业技术体系资助项目(CARS-08-D-3)。

本校为第一机构

以碱性溶液为提取剂,运用单因素试验及响应面法对马铃薯液态培养基培养冠突散囊菌(Eurotium cristatum)所产的胞外黑色素的提取工艺条件进行优化,得到最优提取条件.单因素试验以NaOH溶液浓度、提取温度、色素酸沉pH值和料液比(发酵液∶NaOH溶液)为评价因素,黑色素提取量为响应值,并在此基础上建立4因素3水平响应面试验.结果 表明,冠突散囊菌胞外黑色素的最佳提取工艺参数为:NaOH浓度1 mol/L,提取温度74℃,色素酸沉pH值2.5,料液比为1∶1.4(V∶V).在该优化条件下,提取的黑色素含量最高,为3.982 mg/mL.

Using alkaline solution as extractant,the extraction conditions of extracellular melanin from Eurotium cristatum were optimized by response surface methodology,and the optimal extraction conditions were obtained.On the basis of single factor experiments,using NaOH solution concentration,extraction temperature,pigment acid deposition pH and material to water ratio(fermentation broth∶NaOH ratio)as evaluation factors,the extraction content of melanin as response value,the four factors and three levels of response surface methodology were establis...