期刊论文

Optimization of high density culture conditions of Lactobacillus casei LZ183E

中文

中国酿造

0254-5071

2020

39

12

64-68

10.11882/j.issn.0254-5071.2020.12.013

湖南省自然科学基金(2020JJ4369) 湖南省科技攻关计划(2017GK4032)。

本校为第一机构

为提高干酪乳杆菌LZ183E在发酵培养液中的菌体密度,首先通过在不同温度下培养菌株,测定48 h内菌液的OD600 nm值并作出生长曲线,得到菌株最适培养温度为37℃、接种时间为16 h及收获时间30 h。随后通过单因素试验和正交试验优化,探究不同碳源、氮源、生长因子、初始pH值以及接种量对菌株LZ183E活菌数和OD600 nm值的影响。结果表明,菌株LZ183E的最佳培养条件为葡萄糖25 g/L、酵母膏20 g/L、南瓜汁24 g/L、初始pH 6.5以及接种量2%。此优化条件下,干酪乳杆菌LZ183E的活菌数对数值达到了9.20±0.04,满足高密度培养要求,并且比原始MRS培养基活菌数对数值（8.12±0.06）高出一个数量级,为干酪乳杆菌LZ18...

In order to increase the cell density of Lactobacillus casei LZ183E in the fermentation liquid,first,by cultivating the strains at different temperatures,the OD600 nm value of the bacterial solution within 48 h was measured and the growth curve was drawn,and the optimal culture temperature,inoculation time and harvest time of the strain was obtained,which was 37℃,16 h and 30 h,respectively.Then the effects of different carbon sources,nitrogen sources,nutritional factors,initial pH value and inoculum on the viable count and OD600 nm value of st...