期刊论文

中文

中国酿造

0254-5071

2020

39

8

37-41

10.11882/j.issn.0254-5071.2020.08.008

国家自然科学基金面上项目（31571819）；

本校为第一机构

从浏阳豆豉发酵过程中分离产高酶活菌株,通过形态观察结合分子生物学技术进行鉴定,并对其产蛋白酶、脂肪酶及纤维素酶的活性进行分析。结果表明,分离得到3株菌（编号为000、5132、621）均被鉴定为溜曲霉菌（Aspergillus tamarri）。3株菌的蛋白酶、脂肪酶及纤维素酶的活性测定结果表明,菌株621蛋白酶活性最强,为（207.98±3.20）U/m L;菌株5132的纤维素酶活性最强,为（3.40±1.40）U/m L;菌株000的脂肪酶活性最高,为（90.7±0.64）U/m L。

The strains with high enzyme activity were isolated during Liuyang Douchi fermentation,and identified by morphological observation and molecular biological technique,and the activities of protease,lipase and cellulase were analyzed.The results showed that the 3 isolated strains(No.000,5132,621)were identified as Aspergillus tamarri.The determination results of the protease,lipase and cellulase activities of the 3 isolated strains showed that the protease activity of the strain 621 was the highest of(207.98±3.20)U/ml,the cellulase activity of t...