期刊论文

Determination of 45 pesticide residues in sorghum by QuEChERS combined with gas chromatography triple quadrupole tandem mass spectrometry

中文

中国酿造

0254-5071

2022

41

7

213-219

10.11882/j.issn.0254-5071.2022.07.037

:湘潭市乡村振兴 SF-YB20211001:科技惠民科技项目

本校为第一机构

该实验建立气相色谱三重四极杆串联质谱法（GC-MS/MS）结合改良的QuEChERS方法同时检测高粱中45种农药残留。高粱粉末加水浸润后，加入10 m L乙腈-乙酸乙酯（1∶1,V/V）溶液，加入WondaPak提取盐试剂包Ⅱ提取，取上清液加入WondaPak净化管Ⅲ净化，氮吹近干，定容至1 m L，基质内标法定量。在质量浓度为0.05～1.00μg/m L范围内，各农药的线性关系良好，线性回归方程的相关系数（R2）均＞0.995，方法检出限为0.001 2～0.042 4 mg/kg。分别向阴性高粱样品中添加质量浓度为0.01 mg/kg、0.10 mg/kg和0.50 mg/kg的45种农药标准品，其平均加标回收率为76.32%～108.45%，精密度实验结果的相对标准偏差（...

A gas chromatography triple quadrupole tandem mass spectrometry(GC-MS/MS) method combined with an improved Qu ECh ERS method was established for the simultaneous detection of 45 pesticide residues in sorghum. After the sorghum powder was soaked in water, 10 ml acetonitrileethyl acetate(1∶1, V/V) solution and the Wonda Pak extraction salt reagent pack Ⅱ were added for extraction, the Wonda Pak purification tube Ⅲ was added to supernatant for purification, the nitrogen was blown to nearly dry, the volume was fixed to 1 ml, and the matrix inter...