This work was funded by National Natural Science Foundation of China (31572005) and Research Foundation of Education Bureau of Hunan Province, China (15A090).
机构署名：
本校为第一且通讯机构
院系归属：
园艺园林学院
植物保护学院
摘要：
The southern rice black-streaked dwarf virus (SRBSDV) causes significant economic damage to rice crops. This virus is transmitted to rice plants by the planthopper Sogatella furcifera (Horváth) in a persistent, circular, and propagative manner. Researchers currently lack suitable methods for assaying the activity of SRBSDV in vitro and preserving the virus all year. We used a microinjection method to directly inject SRBSDV extracts into the hemocoel of S. furcifera nymphs. SRBSDV was subsequently detected by Reverse Transcription-Polymerase Ch...
