Methanosarcineae is anaerobic ancient bacterium which can use many kinds of organic or inorganic compounds such as acetic acid,CO2 ,methanol, methylamine, methyl sulfide and so on as the substrate to pro- duce methane. Methanosarcineae has diversity pathway metabolism and simple genetic structure. Methanosar- eineae has been widely used in theoretical and application research. As completion of the whole-genome sequen- cing of the M. acetivorans,M, mazei and M. barkeri, they have become mode strains for studying ancient bacte- ria. In anaerobic digestion,Methanosarcineae as the important strain...