Organic solvents,including weak acid, phenol, alcohol, produced in the conversion process oi olo- mass to biofuels,will harm to the growth of microorganisms, damage the activities of enzyme,and restrict the industrial development at last. But, few microorganisms obtain the abilities to tolerate the high concentration of organic solvents in their evolution process, therefore, they have great potential in future industrial application. This review mainly introduced the tolerance mechanisms of microorganisms to organic solvents, including mem- brane variation and modification, efflux pump, heat s...