期刊论文

中文

化学与生物工程

1672-5425

2015

2

36-39

10.3969/j.issn.1672-5425.2015.02.009

91317312:国家自然科学基金 11K032:湖南省高等学校创新平台开放基金 CX2012B286:湖南省研究生科研创新项目

本校为第一机构

以吲哚-3-乙酸（indole-3-acetic acid,IAA）为模板、甲基丙烯酸为功能单体、乙腈为致孔剂,采用本体聚合法合成了对IAA有特异性识别的吲哚-3-乙酸分子印迹聚合物（IAA-MIP）。红外光谱分析表明,IAA是以氢键形式结合在聚合物的空腔中。等温吸附线及Scatchard分析表明,洗脱模板后的IAA-MIP结合IAA的能力比空白印迹聚合物（NIP）强,且有2种结合方式,其解离常数分别为KD1=1.04×10-6 mol·L-1和KD2=9.23×10-6 mol·L-1,最大表观结合位点分别为Bmax1=0.10μmol·g-1和Bmax2=0.28μmol·g-1。对植物样品的固相萃取实验表明,洗脱模板后的IAA-MIP对IAA具有较强的特异性吸附能力。

Using indole-3-acetic acid(IAA)as template and methacrylic acid as functional monomer and ace-tonitrile as porogen,indole-3-acetic acid molecular imprinted polymers(IAA-MIP)that had specific recognition to IAA were synthesized by bulk polymerization.Infrared spectroscopy indicated that IAA was hydrogen bond-ed in the cavity of the polymers.Adsorption isotherm and Scatchard analysis showed IAA-MIP removed tem-plate had higher affinity to IAA than non-imprinted polymers(NIP).There were two binding modes between IAA-MIP and IAA,the dissociation co...