期刊论文

中文

化学与生物工程

1672-5425

2016

33

3

43-47

10.3969/j.issn.1672-5425.2016.03.012

湖南省环保厅资助项目（湘财建指[2014]287号）； 湖南农业大学东方科技学院大学生研究性学习和创新性实验计划项目（DFCXY201308）；

本校为第一机构

从重金属镉污染土壤中分离筛选出一株耐镉能力为25 mmol· L-1的细菌,根据形态学观察、生理生化实验和分子鉴定,确定为鞘氨醇单胞菌属(Sphingomonas sp.),命名为CdTB02.该菌株的最佳生长条件为:NaCl浓度1％、装液量30 mL/250 mL、pH值6.0、温度35℃;而在含1 mmol·L-1 Cd2+的环境中,最佳耐镉生长条件为:NaCl浓度1％、装液量30mL/250mL、pH值7.0、温度30℃.镉吸附实验结果显示,菌株CdTB02在最佳耐镉生长条件下对Cd2+的吸附率达到94％以上,表明菌株CdTB02对治理镉污染能发挥一定的作用.

In this paper,a strain with maximum cadmium tolerated concentration of 25mmol·L-1 was isolated from the soil of heavy metal cadmium polluted area.It was identified as Sphingomonas sp.and named as CdTB02 by morphological observation,physiological-biochemical identification and molecular identification.The optimum growth conditions for strain CdTB02 were as follows：NaCl concentration of 1%,liquid loading of30mL/250 mL,pH value of 6.0and temperature of 35 ℃.In the environment of containing 1 mmol·L-1Cd2＋,the optimum growth conditions of cadmi...