A reversed-phase high performance liquid chromatographic method was established for simultaneous determination of curcumin, demethoxycurcumin and bidemethoxycnrcumin in the rhizome of Curcuma longa L., a typical herbaceous plant. A Kromasil(TM) C-18 column was used with a mixture of acetonitrile and phosphoric acid aqueous solution ( pH = 2. 5) as the mobile phase. The flow rate was 0. 9 mL/min and the detection wavelength was set at 425 nm. The three constituents, were separated within 10 min. The method is rapid, simple and accurat...
