Swelling of Serum-Stable DNA Nanoparticles upon Target-Induced Conformational Rearrangement of Sensing Probes for the Signal-On Detection of Cancer-Related Genes
National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [21775024, 22174020]; Key Project of Natural Science Foundation of Fujian ProvinceNatural Science Foundation of Fujian Province [2019J02005]
机构署名：
本校为其他机构
院系归属：
生物科学技术学院
摘要：
Nuclease-resistant assay probes are of significant importance for biochemical analysis and disease diagnosis. In this contribution, a reconfigurable lipidic moiety-attached DNA nanoparticle (LDN) is constructed from a cholesterol-conjugated multifunctional hairpin-type DNA probe (Chol-DP) by hydrophobicity-mediated self-assembly. The LDN holds high serum stability and displays a low false-positive signal even in a complex biological milieu. The hydrophobic cholesterol moiety enables the hydrophobicity-mediated assembly, while hydrophilic DNA sequence serves as a recognition element and a polym...
