期刊论文

中文

生物工程学报

1000-3061

1998

14

3

314-319

10.13345/j.cjb.1998.03.014

湖南省教委课题资助；

本校为第一机构

用水稻幼穗为外植体进行组织培养，研究了影响其体细胞胚胎发生的有关因素，建立了高频率体细胞胚胎发生的培养程序。结果表明不同基因型之间体细胞胚胎发生率的差异达到６１２％；生长素２，４Ｄ对诱导水稻体细胞胚起重要的调控作用，细胞分裂素ＢＡ有一定的协同促进作用。干燥处理可提高愈伤组织体细胞胚胎发生率，愈伤组织含水量在６０％～８０％范围的培养效果较好；外源ＤＮＡ溶液浸泡发育早期的心状体细胞胚，其进一步发育时异常胚的频率显著增加，成苗率降低。

The resultes about the influence factors of somatic embryogenesis of rice by culturing the young spikelet explant from 16 varieties.The culture diagram to produce high frequency somatic embryogenesis was established.The resultes indicated:Genotype,horomone,subculture time,treatment of desiccation and exogenous DNA had obivious effects on the somatic embryogenesis and development of rice callus derived from spikelet.The difference of somatic embryogenesis percentage ammony the different varieties was up to 61.2%.Horomone 2.4 D had important role...