In recent years, the exogenes in the transgenic plants being rarely expressed effectively has been universally existed, resolution of this crux is of great significance. In this paper the methods of enhancing and optimizing expression of exogenes were reviewed. These methods include: reselection and reconstruction of promoters; modification of target genes; construction of expression plasmid vector containing MARs; institution of integration site- specific recombinant system; making use of organella- specific signal sequence; adoption of chloroplast transgenic system. In addition, the disadvan...