期刊论文

Lei, H.

中文

生物工程学报

1000-3061

2008

24

5

898-902

10.3321/j.issn:1000-3061.2008.05.029

国家自然科学基金项目（No.30671548）资助~~；

本校为第一且通讯机构

为建立圆弧青霉毒素-青霉酸的免疫学检测方法,研究了青霉酸（PA）的人工抗原合成。通过碳二亚胺法将青霉酸（PA）分别与牛血清白蛋白（BSA）和卵清蛋白（OVA）联结,得到青霉酸人工抗原PA-BSA和PA-OVA。采用紫外扫描光谱法、SDS-PAGE和动物免疫试验对合成的抗原进行鉴定。结果显示联结后的人工抗原特征性吸收峰出现偏移,PA与BSA的偶联比为23.2：1,PA与OVA的偶联比为10.4：1。以PA-BSA为免疫抗原免疫小鼠,PA-OVA为包被抗原,采用间接ELISA检测抗血清,其效价达到1：12800。表明青霉酸的人工抗原已合成,为建立有效的免疫检测方法提供了基础。

To establish a new immune assay for Penicillic Acid (PA) from Penicillium cyclopium, we studied the synthesis of conjugated complete antigens for penicillic acid. PA was conjugated to bovine serum album (BSA) and ovalbumin (OVA) by 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide hydrochloride (EDC). The artificial antigens PA-BSA and PA-OVA were identified by ultraviolet spectrometric scanning, SDS-PAGE and immunization. Results showed that the absorption peak of conjugation were different from that of the carrier protein alone and of the PA. T...