期刊论文

中文

中国生物工程杂志

1671-8135

2009

29

6

63-67

10.13523/j.cb.20090612

浙江省自然科学基金（Y205579）资助项目；

本校为其他机构

研究三叶半夏内生真菌及其凝集素,旨在为半夏内生真菌及其凝集素的开发利用提供依据.对三叶半夏块茎内生真菌分离、纯化,液体发酵培养代谢产物,无水乙醇提取总蛋白,兔血红细胞检测其凝集活性,筛选出菌株gs1,其总蛋白对兔血红细胞凝集活性显著.使用甘露聚糖-Sepharose 4B亲和层析柱纯化菌株gs1总蛋白,得到凝集素.Brandford法定量检测分析表明,1000 ml gs1发酵培养液中含有9.58 mg 凝集素.SDS-PAGE 电泳分析显示该凝集素为单一条带,分子量约为12 kDa.凝集活性实验表明,该凝集素对兔、大鼠和小鼠的血红细胞具有凝集作用,对兔血红细胞效果最显著;而对人(A＼B＼O＼AB型)和鸡的血红细胞无凝集作用.糖结...

Pinellia ternate （Thunb.） Breit has been widely used as more than two thousands years in China. Endophytic fungus from P. ternate a kind of traditional Chinese herb for and the lectin of the endophytic fungus were investigated in order to study the activity of the endophytic fungus. Endophytic fungus were isolated from P. ternate from different provinces in China. Total proteins were extracted by ethanol solvent from the fermentation products of the endophytic fungus. The agglutination activity of proteins, which were extracted from the endop...