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Genomic analysis of a novel isolate Heliothis virescens ascovirus 3i (HvAV-3i) and identification of ascoviral repeat ORFs (aros)

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成果类型:
期刊论文
作者:
Chen, Zi-Shu;Hou, Dian-Hai;Cheng, Xiao-Wen;Wang, Xing;Huang, Guo-Hua*
通讯作者:
Huang, Guo-Hua
作者机构:
[Chen, Zi-Shu; Huang, Guo-Hua; Wang, Xing] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.
[Chen, Zi-Shu; Huang, Guo-Hua; Wang, Xing] Hunan Agr Univ, Inst Virol, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.
[Hou, Dian-Hai] Weifang Med Univ, Sch Biosci & Technol, Weifang 261053, Shandong, Peoples R China.
[Cheng, Xiao-Wen] Miami Univ, Dept Microbiol, 56 Pearson Hall, Oxford, OH 45056 USA.
通讯机构:
[Huang, Guo-Hua] H
Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.
Hunan Agr Univ, Inst Virol, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.
语种:
英文
期刊:
Archives of Virology
ISSN:
0304-8608
年:
2018
卷:
163
期:
10
页码:
2849-2853
基金类别:
The authors would like to thank Jue Hu, Hai-Zhou Liu and Lei Zhang for their help in virus amplification and data analysis. The authors would also like to acknowledge Bi-Chao Xu from the Core Facility and Technical Support of Wuhan Institute of Virology for the technical assistance in SEM and TEM analysis. We thank Dr. Cecil L. Smith (University of Georgia, U.S.A.) for editing and revising the English language. The authors have declared that no competing interests exist. This article does not contain any studies with human or animal subjects performed by any of the authors.
机构署名:
本校为第一且通讯机构
院系归属:
植物保护学院
摘要:
Ascoviruses are circular double-stranded DNA viruses that infect insects. Herein we sequenced and analyzed the genome of the previously unrecorded ascovirus isolate Heliothis virescens ascovirus 3i (HvAV-3i). The genome size is 185,650 bp with 181 hypothetical open reading frames (ORFs). Additionally, definition based on ascovirus repeated ORFs (aros) is proposed; whereby the 29 aros from all sequenced Ascoviridae genomes are divided into six distinct groups. The topological relationship among the isolates of Heliothis virescens ascovirus 3a is (HvAV-3f, {HvAV-3h, [HvAV-3e, (HvAV-3g, HvAV-3i)]...

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