期刊论文

Shen, GL

英文

Analytical Biochemistry

0003-2697

2003

321

1

89-95

10.1016/S0003-2697(03)00427-5

This work was supported by the National Natural Science Foundation of China (Grant Nos. 29975006, 20075006, 70171055, and 50179011), the Foundation of The National 863 High Technologies Research of China (No. 2001AA644020), and the Foundation of Hunan province (Grant Nos. 01JJY1003 and 01JJY2099).

本校为其他机构

A novel immunosensor based on a multilayer-coated glassy carbon electrode was designed to determine isopentenyl adenosine (iPA) in plants. The multilayer consists of polypyrrole and poly(m-phenylenediamine) with K 4Fe(CN)6 and horseradish peroxidase (HRP) entrapped during electropolymerization. The ferrocyanide doped in polypyrrole functions as the mediator. The glucose oxidase bound on the immunosensor by the competitive immunoreaction involving iPA catalyzed the oxidation of the added glucose with the formation of H2O2, which is in turn reduc...