期刊论文

Analysis and Determination of Ceramide in Konjac by HPLC-ELSD

中文

中国生物化学与分子生物学报

1007-7626

2010

26

2

189-194

10.13865/j.cnki.cjbmb.2010.02.005

湖南省科技计划重大专项（No.04NK1002）~~；

本校为第一机构

建立高效液相色谱-蒸发光散射检测器分析神经酰胺的方法并进行了含量的测定.色谱柱:ZORBZX Eclipse XDB-C18(4.6 mm×250 mm,5μm),洗脱方法:梯度洗脱,柱温:35 ℃,流动相:甲醇/水,流速:1 ml/min;检测器:蒸发光散射检测器,漂移管温度:40℃,氮气流速:1.5 L/min.系统探讨了梯度洗脱的起始浓度、洗脱的时间和洗脱梯度的程序设置,最佳的梯度洗脱条件为5 min内,甲醇浓度从60%线性增加为90%,从5 min到25 min,甲醇浓度线性增加为95%,在此条件下样品和标准品的分离色谱峰对称性较好.随后测定了各种样品中神经酰胺的含量,并进行了方法学验证,结果神经酰胺在0.2～2μg之间线性关系良好,最低检测限为0.01 mg...

An analytical method using high-performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) has been developed for the determination of ceramide extracted from konjac. The simultaneous separation of ceramide was achieved on a ZORBZX Eclipse XDB-C18 column (4.6 mm×250 mm, 5 μm) by gradient elution with methanol-water as the mobile phase at the flow rate of 1 mL/min and the column temperature at 35 ℃. The parameters for ELSD were set by the drift tube temperature 40 ℃ and nitrogen flow-rate 1.5 L/min. After a s...