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A robust analytical method for measurement of phytoestrogens and related metabolites in serum with liquid chromatography tandem mass spectrometry

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成果类型:
期刊论文
作者:
Jiang, Hongmei;Liao, Xiangjun;Wood, Carla M.;Xiao, Chao-Wu*;Feng, Yong-Lai*
通讯作者:
Feng, Yong-Lai;Xiao, Chao-Wu
作者机构:
[Jiang, Hongmei] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.
[Feng, Yong-Lai; Jiang, Hongmei; Liao, Xiangjun] Hlth Canada, Exposure & Biomonitoring Div, Environm Hlth Sci & Res Bur, Hlth Environm & Consumer Safety Branch, 50 Columbine Driveway,AL 0800C, Ottawa, ON K1A 0K9, Canada.
[Wood, Carla M.; Xiao, Chao-Wu; Jiang, Hongmei] Hlth Canada, Hlth Prod & Food Branch, Nutr Res Div, 251 Sir Frederick Banting Driveway, Ottawa, ON K1A 0K9, Canada.
通讯机构:
[Feng, Yong-Lai; Xiao, Chao-Wu] H
Hlth Canada, Exposure & Biomonitoring Div, Environm Hlth Sci & Res Bur, Hlth Environm & Consumer Safety Branch, 50 Columbine Driveway,AL 0800C, Ottawa, ON K1A 0K9, Canada.
Hlth Canada, Hlth Prod & Food Branch, Nutr Res Div, 251 Sir Frederick Banting Driveway, Ottawa, ON K1A 0K9, Canada.
语种:
英文
关键词:
Biomolecules;Body fluids;Chromatography;Column chromatography;Flavonoids;High performance liquid chromatography;High pressure liquid chromatography;Liquids;Mass spectrometry;Metabolites;Rats;Shells (structures);Signal to noise ratio;Spectrometry;Core shell;High-performance liquid chromatography tandem mass spectrometries;Phytoestrogens;Rapid separation;Rat serum;Liquid chromatography;beta glucuronidase;casein;equol;glucuronidase H 5;glycitein;isoflavone derivative;phytoestrogen;soybean protein;sulfatase;sulfatase H 1;unclassified drug;phytoestrogen;animal experiment;Article;calibration;controlled study;female;Helix pomatia;high performance liquid chromatography;hormone determination;hormone release;limit of detection;metabolomics;nonhuman;priority journal;quantitative analysis;rat;signal noise ratio;tandem mass spectrometry;animal;blood;chemistry;high performance liquid chromatography;isolation and purification;procedures;Sprague Dawley rat;tandem mass spectrometry;Animals;Chromatography, High Pressure Liquid;Female;Limit of Detection;Phytoestrogens;Rats;Rats, Sprague-Dawley;Tandem Mass Spectrometry
期刊:
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN:
1570-0232
年:
2016
卷:
1012-1013
页码:
106-112
基金类别:
This project was financially supported by the Canadian Government under the Chemicals Management Plan.
机构署名:
本校为第一机构
院系归属:
理学院
摘要:
A sensitive and robust method using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed for quantitation of 13 phytoestrogens and related metabolites in rat serum samples. A new type of column, the Kinetex core-shell C18 column, was applied for rapid separation of the target analytes in 10 min. Two enzymes, sulfatase H-1 and gulcuronidase H-5 from Helix pomatia were compared on the efficiency of releasing the conjugated forms of the target analytes to their free forms in serum samples. The method detection...

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