期刊论文

中文

广东农业科学

1004-874X

2012

39

14

74-77

10.16768/j.issn.1004-874x.2012.14.006

Key technology research and demonstration of comprehensive control on herbicide phytotoxicity in southern tobacco area（SR0558）；

本校为第一机构

通过富集培养技术结合高效液相色谱法检测,从长期受丁草胺污染的污泥中筛选出1株丁草胺降解菌,命名为Y-1。经形态特征、生理生化特征和16S rDNA序列同源性分析,将该菌株鉴定为假单胞菌属（Pseudomonas sp.）。通过研究Y-1在不同条件下的降解特性发现,降解丁草胺的最优条件为：丁草胺初始浓度20 mg/L、接种量5%、pH值7、培养温度30℃。在最优环境条件下培养7 d,Y-1能降解培养液中76%的丁草胺,显示了良好的降解能力。

From the sludge polluted by butachlor for a long time,the degrading bacterium to butachlor was selected through enrichment culture with combination of High Performance Liquid Chromatography(HPLC) detection and then named as Y-1.Through the analysis of morphological characteristics,physiological and biochemical characteristics,and 16S rDNA sequence homology,Y-1 was identified as Pseudomonas sp..Through the research of the degradation character of Y-1 in different ambient conditions,the optimal conditions were found as follows: the initial concen...