期刊论文

Liu, Q.

中文

遗传

0253-9772

2008

30

9

1195-1200

10.3321/j.issn:0253-9772.2008.09.016

国家自然科学基金项目（编号：30600049）,湖南省科技应用基础研究计划重点项目（编号：2007FJ3003）和湖南省科技计划项目（编号：06FJ3079）资助.致谢：感谢中国科学院上海植物生理与生态研究所壬宗阳研究员为本研究提供的遗传转化载体.

本校为第一机构

以单质粒转化的粳稻广粳一号和双质粒共转化的籼稻01早5202所获得的转反义蜡质基因水稻后代为供试材料,通过潮霉素抗性、PCR检测等手段分析了外源基因的遗传分离特性,同时,还分析了转基因材料的直链淀粉含量、waxy蛋白含量的变化特性。结果表明,无论是采用标记基因（hpt）与目的基因（Anti-sense waxy）连锁的单质粒转化,还是采用双质粒共转化,其供试的后代植株材料都发生了外源基因分离现象,且转基因植株材料的直链淀粉含量都有所下降,有些单株的直链淀粉含量已降至10%（质量百分比）以下,远低于对照（其直链淀粉含量为22.04%）;SDS-PAGE检测结果显示,供试的转基因材料的waxy蛋白的含量与对应的...

The segregation of exogenous genes was studied by hygromycin-resistant and PCR experiments in the transgenic rice (Oryza sativa L. subsp. japonica and indica) with anti-sense waxy gene, meanwhile the change of amylose and waxy protein contents in progenies of transgenic rice was analyzed. The results showed no matter the rice Guangjing No.1 (O. Sativa L. subsp. japonica) were transformed by p13w4 plasmid carrying anti-sense waxy gene and hygromycin-resistant gene, or in the rice 01Z5202 (O. sativa L. subsp. indica) were co-transformed by p13w8 plasmid carrying anti-sense waxy gene and p1300 pl...