期刊论文

Progress on genome-wide CRISPR/Cas9 screening for functional genes and regulatory elements

中文

遗传

0253-9772

2020

42

5

435-443

10.16288/j.yczz.19-390

湖南省生猪产业技术体系岗位专家项目(编号:2019-2021) 深圳市技术攻关项目(编号:JSGG20180507182028625) 转基因生物新品种培育重大专项(编号:2016ZX08006002-005) 广东省重点领域研发计划(现代种业)项目(编号:2018B020203002)资助。

本校为第一机构

CRISPR/Cas9系统是一种近年来被广泛应用于基因组编辑的强大工具。通过将CRISPR/Cas9系统中的Cas9蛋白突变后,使其失去剪切活性而成为dCas9 (nuclease-dead Cas9),再结合基因功能丧失(loss-of-function, LOF)、基因功能激活(gain-of-function, GOF)以及非编码功能基因鉴定技术即可实现全基因组高通量的功能基因及调控元件靶向鉴定和筛选。目前,该技术已被广泛应用于疾病免疫机理、药物靶点筛选和动物遗传育种等研究,为生命医学和基础科学带来了全新高效的技术方法和研究思路。本文综述了基于CRISPR/Cas9技术在全基因组中高通量筛选功能基因及调控元件的方法及研究进展,重点阐述了CRISPR/Cas9系统...

The CRISPR/Cas9 system is a powerful tool which has been extensively used for genome editing in the past few years. Nuclease-dead Cas9 (CRISPR/dCas9), a Cas9 protein mutant without splicing ability, along with loss-of- function (LOF), gain-of-function (GOF), or non-coding genes scanning approaches can reveal genome-scale functional determinants. CRISPR/Cas9 has been widely adopted to decipher disease mechanisms and pinpoint drug targets in the life science field, and also provide novel insights into animal genetics and breeding. In this review, we summarize the research progress in high-throug...