期刊论文

Effects of Different Phosphorus Levels on Na~＋/PiⅡb Transport Proteins mRNA Expression and Phosphorus Uptake

中文

动物营养学报

1006-267X

2010

22

4

1000-1006

10.3969/j.issn.1006-267x.2010.04.028

湖南省自然科学基金“离子依赖型磷转运载体蛋白对磷吸收的影响”(08JJ3084)； 湖南省教育厅项目“Na+/PiⅡb型协同转运蛋白在小肠中的表达量与磷吸收的关系”(08C42500)；

本校为第一机构

本文通过大鼠饲喂试验研究了不同磷水平对磷吸收的影响,旨在进一步揭示无机磷的吸收机理.试验选用120只体重相近的昆明大白鼠,随机分成4个处理,每处理5个重复,每个重复6只大白鼠,分别饲喂含磷(总磷)0.2%、0.4%、0.6%、0.8%的饲粮,试验期14d,测定血钙血磷、骨钙骨磷及不同肠段Na+/PiⅡb载体转运蛋白mRNA表达量和磷的吸收等指标.结果表明,0.2%低磷组回肠、空肠、十二指肠Na+/PiⅡb载体转运蛋白mRNA表达量分别是0.6%磷水平组的3.10(P

A rats feedig trial was conducted to investigate the effects of phosphorus levels on phosphorus uptake to reveal the mechanism of phosphate（Pi）uptake.One hundred and twenty male Kunming rats with body weight of 18 to 22 g were randomly divided into 4 treatments,and each treatment consisted of 5 replicates with 6 rats per replicate.The 4 treatments were fed the basal diets supplemented with 0.2%,0.4%,0.6% and 0.8% total phosphate.The experiment lasted for 14 days.Calcium（Ca）and Pi concentration in bone and serum and Na＋/PiⅡb mRNA expressio...