期刊论文

中文

动物营养学报

1006-267X

2012

24

10

2021-2027

10.3969/j.issn.1006-267x.2012.10.023

国家自然科学基金资助项目（30972118）； 湖南省自然科学基金资助项目（08JJ3080）；

本校为第一机构

为研究大豆异黄酮(soybean isoflavones,SI)对香猪睾丸形态及精子发生标志基因表达的影响,选择40头健康的28日龄雄性香猪,随机分成4组,每组10个重复,每个重复1头猪.1、2、3和4组分别在基础饲粮中添加0、125、250和500 mg/kg SI,饲喂60 d后从各组分别随机选取5头屠宰,采取睾丸样品,分析睾丸的组织形态及E型钙粘连蛋白1(Cdh1)、联会复合体蛋白3(SCP3)、过渡蛋白1(Tnp1)和波形蛋白(Vim)基因的表达.结果表明:各添加水平的SI均造成了睾丸曲细精管中空泡的形成,减少了管腔中成熟精子的数量；饲粮添加SI极显著降低了睾丸组织Cdh1、SCP3和Tnp1基因的表达量(P＜0.01)；添加250 mg/kg的SI显著降低了睾丸组...

To investigate the effects of soybean isoflavones(SI) on testis morphology and expression of marker genes during spermatogenesis of Xiang pigs,40 healthy male pigs aged 28 days were selected and divided randomly into 4 groups with 10 replicates in each group and 1 pig per replicate.Pigs in groups 1,2,3 and 4 were fed a basal diet supplemented with 0,125,250 and 500 mg/kg SI,respectively.Five pigs in each group were slaughtered after 60 d of feeding,and the tissue of testis was sampled for the analysis of tissue morphology and gene expression of Cdh1,SCP3,Tnp1 and Vim.The results showed as foll...