期刊论文

中文

动物营养学报

1006-267X

2014

5

1379-1386

10.3969/j.issn.1006-267x.2014.05.032

湖南省自然科学基金（14JJ4039） 湖南省发改委试验项目（[2013]766）

本校为第一机构

本试验旨在探讨饲料中添加α-酮戊二酸（ AKG）对草鱼热休克蛋白70（ HSP70）和过氧化物酶体增殖物激活受体γ（PPARγ）mRNA表达的影响。选取平均体重为（35．66±0．39） g的健康草鱼450尾，随机分为5组（每组3个重复，每个重复30尾鱼）：对照组饲喂基础饲料，试验组分别饲喂以0．25％、0．50％、0．75％、1．00％AKG等量替换基础饲料中次粉的试验饲料。试验期为60 d，试验结束后采用实时荧光定量PCR方法检测草鱼肝胰脏、鳃、肾脏及脾脏中HSP70和PPARγmRNA的相对表达量。结果表明：HSP70和PPARγmRNA的相对表达量在不同组织中存在差异，二者均在肾脏中高度表达，HSP70 mRNA的相对表达量在肾脏...

This experiment was conducted to investigate the effects of α-ketoglutarate ( AKG) on heat shock protein 70 ( HSP70 ) and peroxisome proliferator activated receptor γ( PPARγ) mRNA expressions of grass carp (Ctenopharynodon idellus).A total of 450 grass carp with an average body weight of (35.66±0.39) g were randomly allotted to 5 groups with 3 replicates per group and 30 fish per replicate.Fish in control group were fed a basal diet, while those in experimental groups were fed experiemental diets supplemented with 0.25%, 0.50%, 0.75%and 1.0...