期刊论文

中文

动物营养学报

1006-267X

2016

28

7

2090-2095

10.3969/j.issn.1006-267x.2016.07.013

31301985:国家自然科学基金 2014M562111:中国博士后科学基金 2015T80871:中国博士后科学基金 2015RS4035:湖南省科技厅项目

本校为第一机构

本试验旨在通过脂多糖(LPS)诱导猪空肠上皮细胞(IPEC-J2)来建立氧化应激模型,探讨壳寡糖(COS)的抗氧化作用效果。采用噻唑蓝(MTT)法检测LPS和COS对IPEC-J2作用12、24、48 h后细胞增殖活性的变化;选择适宜浓度LPS(1.0 μg /mL)和COS(200 μg /mL)作用于IPEC-J2,分为对照组、LPS组、COS组、LPS+COS组。采用Western Blot法检测核因子E2相关因子2(Nrf2) 、血红素氧合酶-1(HO-1)蛋白的表达;试剂盒检测超氧化物歧化酶(SOD) 、过氧化氢酶(CAT)活性及丙二醛(MDA)的含量。结果表明: 1.0 μg /mL的LPS对IPEC-J2作用24 h,细胞增殖的抑制率为41.6%,为造模的最适浓度和作用时间; COS在一定浓度范围内对IPEC-J...

This study was designed to investigate the effect of chitooligosaccharide ( COS) on oxidative stress and used lipopolysaccharide ( LPS) induced epithelial cells of pig jejunum ( IPEC?J2) as an in vitro model of oxidative stress. The IPEC?J2 were incubated with LPS and COS for 12, 24 and 48 h,and then used methylthi?azolyldiphenyl?tetrazolium bromide ( MTT) method to detect the activity of cell proliferation; the appropriate concentrations of LPS (1.0 μg/mL) and COS (200 μg/mL) were chosen in the IPEC?J2 cells, divided into control group, LPS ...