期刊论文

Resveratrol Attenuates Acute Oxidative Injury in Mouse Leydig Cell TM3 via Silent Information Regulator 1 /Uncoupling Protein 2 Signaling Pathway

中文

动物营养学报

1006-267X

2018

30

7

2632-2640

10.3969/j.issn.1006-267x.2018.07.023

31772819:国家自然科学基金 31572591:国家自然科学基金 2014T70770:中国博士后科学基金特别资助项目(第七批)

本校为第一机构

本试验旨在研究白藜芦醇( RES)对氧化损伤小鼠睾丸间质细胞TM3的保护作用,并探索其可能的作用机制。首先,用不同浓度( 0、150、200、250、300 μmol /L)的过氧化氢( H_2O_2 )处理小鼠睾丸间质细胞TM3 8 h,确定建立氧化损伤细胞模型的适宜H_2O_2浓度;然后,用不同浓度( 0、2.5、5.0和10.0 μmol /L)的RES分别处理正常细胞24 h,确定RES安全浓度;最后,用安全浓度的RES处理氧化损伤细胞24 h。在整个培养过程中采用iCELLigence实时无标记细胞功能分析仪监测细胞的增殖情况;待安全浓度的RES处理氧化损伤细胞结束后采用2',7'-二氯荧光素二乙酸酯( DCFH-DA)探针法检测细胞中活性氧( ROS)的含量,实时荧光定...

This experiment was conducted to investigate the protective effect of resveratrol ( RES) on oxidativedamaged mouse Leydig cell TM3,and to explore its possible mechanism. Firstly,TM3 cells were treated with different concentrations ( 0,150,200,250 and 300 μmol /L) of hydrogen peroxide ( H_2O_2 ) ,and the optimal concentration of H_2O_2 was selected to establish the oxidative-damaged cell model. Secondly,the normal cells were treated with different concentrations ( 0,2.5,5.0 and 10.0 μmol /L) of RES for 24 h to select the safe concentration of ...