期刊论文

中文

动物营养学报

1006-267X

2018

30

12

5083-5088

10.3969/j.issn.1006-267x.2018.12.036

湖南省自然科学基金（10JJ5015）；

本校为第一机构

本试验以猪小肠上皮细胞( IPEC-1)为模型,探讨牛膝多糖( ABPS)对IPEC-1增殖、紧密连接相关蛋白mRNA表达和沙门氏菌侵染的影响.在IPEC-1细胞培养基中分别加入相应剂量的ABPS,使培养基中ABPS终浓度分别为0(对照) 、50、100、200、400 μg /mL.分别采用噻唑蓝( MTT)法、实时荧光定量PCR法和平板细菌计数法检测ABPS对IPEC-1的影响.结果表明: 1)与对照组相比,ABPS对IEPC-1增殖无显著影响( P>0.05) .2) ABPS处理能显著抑制沙门氏菌的侵染,随着ABPS浓度的增加,其抑制效果先增加后减少,浓度为50 μg /mL时抑制效果达到峰值,其细胞被侵染的沙门氏菌数量极显著低于对照组和200、400 μg /mL ABPS组( P< 0....

This experiment was conducted to evaluate the effects of Achyranthes bidentata polysaccharides(ABPS)on proliferation,mRNA expression of tight junction related proteins and infection of Salmonella in intestinal porcine epithelial cells-1(IPEC-1).Adding ABPS in IPEC-1 culture medium,the final concentration of ABPS were 0(control),50,100,200,400μg/mL.The effects of ABPS on IPEC-1 were detected using MTT assay,quantitative real-time PCR and plate colony counting method.The results showed as follows:1)compared with the control group,ABPS had no sig...