期刊论文

Tannic Acid Induces Oxidative Damage and Apoptosis in Pig Kidney Cells

中文

动物营养学报

1006-267X

2020

32

9

4327-4336

10.3969/j.issn.1006-267x.2020.09.043

国家重点研发计划资助项目（2016YFD0501209）；

本校为第一机构

本试验对单宁酸( TA)介导的动物肾细胞毒性的分子机制展开研究,旨在为消减和利用饲料中的抗营养因子提供试验依据。采用猪肾细胞系PK-15细胞,以不同浓度[ 0(空白对照) 、10、20、30 μmol / L] TA处理PK-15细胞24 h后,测定细胞活性、乳酸脱氢酶( LDH)活性、氧化损伤指标、线粒体膜电位( MMP) 、凋亡相关蛋白表达量、细胞周期。结果显示:TA剂量依赖性地降低PK-15细胞的活力。与空白对照相比,30 μmol / L TA极显著增加了培养基中LDH的活性( P

The aim of this study was to explore the molecular mechanism of tannic acid (TA) mediated renal cytotoxicity in animals, and to provide experimental basis for the reduction and utilization of antinutritional factors in feed. Pig kidney cell line PK-15 cells were treated with different concentrations of TA [0 (blank control), 10, 20 and 30 μmol / L] for 24 h. Cell viability, lactate dehydrogenase (LDH) activity, oxidative damage parameters, mitochondrial membrane potential (MMP), apoptosis related protein expression and cell cycle were measured...