期刊论文

Extraction of Polyphenols from Mulberry Leaves and Its Determination of Antioxidant Capacity in Vitro

中文

动物营养学报

1006-267X

2021

33

06

3573-3580

10.3969/j.issn.1006-267x.2021.06.059

国家重点研发计划政府间国际科技创新合作重点专项（2018YFE0101700）； 湖南省科技人才托举工程（2020TJ-Q02）； 湖南省教育厅科学研究项目一般项目（19C0937）；

本校为第一机构

为研究最佳的桑叶多酚提取方法,并综合评价桑叶多酚的体外抗氧化能力,本试验采用浓度分别为60%、70%、80%的乙醇提取桑叶多酚,建立没食子酸标准曲线测定桑叶多酚的绝对量和含量。将各提取组桑叶多酚提取液稀释2、4、8、16倍后测定其1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除率,并以水溶性维生素E(Trolox)为阳性对照,半抑制浓度(IC_(50))为参考依据,对各组提取液及稀释液进行体外抗氧化能力测定。结果表明:1) 60%乙醇提取组的桑叶多酚绝对量和含量最高,但各提取组之间无显著差异(P>0.05) 。2)将60%乙醇提取组中的桑叶多酚提取液分别稀释至原液的25%、50%、75%后测定各稀释组的桑叶多酚绝对量及含...

In order to study the best extraction method of polyphenols from mulberry leaves, and comprehensively evaluate the antioxidant capacity of polyphenols from mulberry leaves, the concentrations 60%, 70% and 80% ethanol were used for extraction of polyphenols from mulberry leaves, respectively, and gallic acid standard curve was established to determine the absolute amount and content of polyphenols from mulberry leaves. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging rate in extracting solution of extraction groups was determined after 2, 4, 8 and 16 times dilution, and the anti...