The experiment was conducted to analyze different corn varieties (normal corn and high amylose corn) and starch retrogradation on feed nutrients, Fourier transform infrared spectroscopy (FTIR) molecular structure and in vitro fermentation parameters, and to explore the correlation between FTIR molecular structure parameters and in vitro fermentation characteristics of corns. The results showed that there were significant interactions between corn variety and starch retrogradation on sectional FTIR structure parameters, total volatile fatty acid...