期刊论文

He, J.

中文

动物营养学报

1006-267X

2023

35

7

4604-4616

10.12418/CJAN2023.428

国家自然科学基金项目(31972600)。

本校为第一机构

本试验旨在研究白藜芦醇对热应激诱导肉鸡脾脏淋巴细胞增殖及凋亡的作用。本试验通过分离和消化白羽肉鸡脾脏组织,成功培养出原代淋巴细胞,并能稳定传代。通过构建正常细胞模型(37℃)和热应激细胞模型(42℃培养3 h),培养鸡脾...展开更多 本试验旨在研究白藜芦醇对热应激诱导肉鸡脾脏淋巴细胞增殖及凋亡的作用。本试验通过分离和消化白羽肉鸡脾脏组织,成功培养出原代淋巴细胞,并能稳定传代。通过构建正常细胞模型(37℃)和热应激细胞模型(42℃培养3 h),培养鸡脾脏淋巴细胞,并分别添加不同剂量的白藜芦醇(15、30、60μg/mL)。运用CCK8法测定热应激对脾脏淋巴细胞存活率的影响;取脾脏淋巴细胞上清液...

This experiment was conducted to study the effects of resveratrol on proliferation and apoptosis of spleen lymphocytes in broilers under heat stress.In this study,primary lymphocytes were successfully cultured by isolating and digesting spleen tissues from white⁃feather...MORE This experiment was conducted to study the effects of resveratrol on proliferation and apoptosis of spleen lymphocytes in broilers under heat stress.In this study,primary lymphocytes were successfully cultured by isolating and digesting spleen tissues from white⁃feather...