期刊论文

中文

浙江农业学报

1004-1524

2022

34

2

337-342

10.3969/j.issn.1004-1524.2022.02.15

湖南省教育厅创新平台项目（19K040）； 湖南省自然科学基金（2018JJ3220）；

本校为第一机构

以番茄匍柄霉(Stemphylium lycopersici)CS12菌株为研究对象,从菌龄、酶种类与浓度、酶组合、酶解温度与时间、不同渗透压稳定剂等方面对番茄匍柄霉原生质体制备条件进行了优化,并筛选了最适再生培养基,成功建立番茄匍柄霉原生质体制备与再生体系。番茄匍柄霉CS12在菌龄为36 h,稳渗剂为0.7 mol·L~(-1) NaCl溶液,酶解液为10 mg·mL~(-1)Kitalase裂解酶, 28 ℃酶解3.5 h的条件下制备的原生质体效果好,在YEPS再生培养基上再生效率最高。番茄匍柄霉原生质体制备与再生体系的建立,为进一步研究番茄匍柄霉致病机理与功能基因组学奠定了基础。

Protoplastpreparation conditions of Stemphylium lycopersici was optimizedusing strain CS 12 fromhypha age,enzyme species and concentration,enzyme combination,enzymolysis temperature and time,isotonic solutions,and the optimal regeneration medium.Theprotoplasm preparation and regeneration of S.lycopersici was successfully established by using mycelium cultured 36 h,0.7 mol·L-1 NaCl solution as the osmotic stabilizer,10 mg·mL-1 Kitalase as enzymatic hydrolysate at 28℃for 3.5 h,and the regeneration efficiency was the highest on the YEPS regener...