期刊论文

英文

International Journal of Molecular Sciences

2023

24

4

10.3390/ijms24043633

Horticulture's Open program of Hunan Agricultural University [2021YYXK002]; Key Scientific Research Fund of Hunan Provincial Education Department [21A0138]; National Natural Science Foundation of China [81903752, 31600238]; Project of Hunan Natural Science Foundation [2022JJ30305]; Project of Guangxi Natural Science Foundation [2019GXNSFBA185026]

本校为第一机构

Protoplast-based engineering has become an important tool for basic plant molecular biology research and developing genome-edited crops. Uncaria rhynchophylla is a traditional Chinese medicinal plant with a variety of pharmaceutically important indole alkaloids. In this study, an optimized protocol for U. rhynchophylla protoplast isolation, purification, and transient gene expression was developed. The best protoplast separation protocol was found to be 0.8 M D-mannitol, 1.25% Cellulase R-10, and 0.6% Macerozyme R-10 enzymolysis for 5 h at 26 degrees C in the dark with constant oscillation at ...