期刊论文

Cheng, TY

英文

Ticks and Tick-borne Diseases

1877-959X

2022

13

2

101892

10.1016/j.ttbdis.2021.101892

This research was supported by a grant from the National Science Foundation for Young Scientists of China (No. 31902294), funds from Agricultural Committee of Hunan Province, China (No. 2019NK2181), a grant from the Scientific and Technological Research Projects of Henan Province, China (No. 172102410051), the Science Fund for Distinguished Young Scholars of Changsha City, China (No. kq2009055) and funds from the Jiangsu Key Laboratory of Zoonosis (No. R1706).

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The full-length cDNA of two ferritins of Haemaphysalis flava were cloned after which recombinant Hf-FER1 and Hf-FER2 were expressed and their function was analyzed. In addition, RNA interference (RNAi) based on the injection of Hf-fer1 or Hf-fer2 dsRNA into fully engorged female ticks was performed. The cDNA encoding Hf-FER1 is 834 bp in length. It contains an iron-responsive element in the 5ʹ untranslated region and encodes 174 amino acid residues. The full-length cDNA of Hf-FER2 contains 696 bp and encodes 199 amino acids, including a putati...