期刊论文

中文

中国科学：生命科学

1674-7232

2016

46

3

274-284

10.1360/N052015-00174

(批准号:CARS-36):国家现代农业产业技术体系建设专项资金 (批准号:CX2015B251):湖南省研究生科研创新项目

本校为第一机构

可变剪接作为一种增加基因组和蛋白质组多样性的重要机制, 广泛发生于真核生物基因转录的过程中.本文采用Illumina Hiseq 2500测序平台对猪睾丸组织4个不同发育时期(60胚龄、90胚龄、30日龄和180日龄)进行转录组测序, 以猪基因组数据为参考, 鉴定和分析了猪基因组可变剪接事件. 从猪基因组中鉴定出20398个基因(80.6%)对应的92738个可变剪接事件.在不同的可变剪接类型中, 以第一个外显子可变剪切(alternative 5′ first exon, TSS)、最后一个外显子可变剪切(alternative 3′ last exon, TTS)、单外显子跳跃(skipped exon, SKIP)和可变5′或3′端剪切(alternative exon ends, AE) 4种类型为主,分别...

Alternative splicing has been widely found in the eukaryotic gene transcription and serves as an important mechanism to enrich the genomic and proteomic diversity. In the current study, we sequenced the transcriptomic profiling of pig testes at four representative stages: 60 dpc (days post coitus, E60), 90 dpc (E90), 30-day-old (D30) and 180-day-old (DM) using Illumina Hiseq 2500 technology. Then, RNA-seq data were further used to identify the alternative splicing events referring the Sus Scrofa genome. The results showed that 92738 alternative...