A molecularly imprinted polymer (MIP) was prepared in acetonitrile by bulk polymerization, using di-n-octylphthalate (DOP) as a template molecular, α-methacrylic acid (MAA) as a functional monomer, and ethylene dimethacrylate (EDMA) as a crosslinker. Characterization and evaluation of the prepared MIP were carried out by scanning electron microscope (SEM), infrared absorption spectroscopy (IR), and the Scatchard analysis, respectively. Through the optimization of washing solvent, eluting solvent amount, flow rate of loading solution, and loadi...