期刊论文

Yao, Bin

英文

Enzyme and Microbial Technology

0141-0229

2010

46

6

506-512

10.1016/j.enzmictec.2010.02.003

Chinese National High Technology Research and Development Program (863 Program)National High Technology Research and Development Program of China [2007AA100601]; National Key Technology R&D Program of ChinaNational Key Technology R&D Program [2006BAD12B05-03]; Key program of Transgenic Plant Breeding [2008ZX003-002]

本校为其他机构

A xylanase gene, xynAM6, was isolated from the genomic DNA library of Streptomyces megasporus DSM 41476 using colony PCR screening method. The 1440-bp full-length gene encodes a 479-amino acid peptide consisting of a putative signal peptide of 36 residues, a family 10 glycoside hydrolase domain and a family 2 carbohydrate-binding module. The mature peptide of xynAM6 was successfully expressed in Pichia pastoris GS115. The optimal pH and temperature were pH 5.5 and 70°C, respectively. The enzyme showed broad temperature adaptability (>60% of th...