With attention to environmental pollution and food safety, molecular imprinted solid phase extraction technique in various fields has been further developed. Therefore, molecular imprinted solid phase extraction were briefly described in this article. Molecular imprinted solid phase extraction enrichment and detection of pesticide residues, drug residues in food and feed, other contaminants in water and soil, and also molecular imprinted solid phase extraction of drug testing and purification of functional pharmaceutical ingredient in recent years were reviewed. In this paper, the problem mole...