期刊论文

Screening of Aerobic Denitrifying Bacteria and Nitrogen Removal Characteristics of Bacteria-algae Co-culture System

中文

环境科学与技术

1003-6504

2021

44

08

154-162

10.19672/j.cnki.1003-6504.0661.21.338

湖南省重点研发项目:池塘养殖水质净化新型微生态制剂研究与开发（2016NK2103）；

本校为第一机构

为提高养殖废水的除氮效果,该文从精养池塘水体中分离出1株高效好氧反硝化细菌TC-1,通过形态学观察、生理生化试验并结合16S r DNA序列分析对反硝化细菌TC-1进行了鉴定。选取椭圆小球藻与反硝化细菌TC-1为研究对象,运用菌藻共培养法研究了碳源种类、总接种量、初始p H值、培养温度、摇床转速对菌藻共培养体系除氮性能的影响,并应用Box-Behnken中心组合进行3因素3水平的实验设计对培养条件进行优化。结果表明,菌株TC-1被鉴定为Pseudomonas toyotomiensis TC-1。菌藻共培养体系适宜的脱氮条件为:在以丁二酸钠为碳源,总接种量2%,初始pH 7.04,培养温度30℃,摇床转速169 r/min的条件下,亚硝氮和总氮去...

In order to improve the nitrogen removal efficiency of aquaculture wastewater, a species of aerobic denitrifying bacterium TC-1 was isolated from the water of intensive aquaculture pond. The denitrifying bacterium TC-1 was identified by means of morphological observation, physiological and biochemical tests and 16S rDNA sequence analysis. In addition, Chlorella ellipsoides was selected, which together with denitrifying bacteria TC-1 as the research objectives, the effects of carbon sources, inoculation amount, initial pH value, culture temperat...