In vitro;Potato;PVA;Thermotherapy;Virus eradications
期刊：
INDIAN JOURNAL OF AGRICULTURAL SCIENCES
ISSN：
0019-5022
年：
2016
卷：
86
期：
4
页码：
541-544
基金类别：
Modern Agriculture Industry Technology System of China
机构署名：
本校为第一且通讯机构
院系归属：
园艺园林学院
摘要：
With the aim of developing an effective protocol for virus elimination from potato (Solanum tuberosum L.) plantlets, thermotherapy coupled with isolating the first nodal cuttings by in vitro culture was successful to potato virus A (PVA) elimination. The survival ratio of potato plantlets was affective by thermotherapy temperatures and durations. The optimal thermotherapy temperature was 36±1 oC with highest survival ratio and effective elimination. The results of RT-PCR indicated that the regenerated plantlets obtained from the first cycle (f...
