期刊论文

中文

华北农学报

1000-7091

2014

29

3

52-58

863;2012AA101107-3:国家高技术研究发展计划（863计划） ZWKF201303:湖南农业大学作物学科开放基金

本校为第一机构

为进一步研究油菜LPAT4基因,通过检索与拟南芥LPAT4序列相似度较高的油菜EST序列,拼接后与白菜和甘蓝的LPAT4序列进行同源比对,设计一对引物,从甘蓝型油菜湘油15中扩增全长LPAT4 CDs序列。共获得2条CDs序列,它们的长度分别为1 143,1 140 bp,分别命名为BnLPAT4-1和BnLPAT4-2。BnLPAT4-1编码380个氨基酸,所得蛋白质的相对分子质量和等电点分别为43.29 kDa和8.74;BnLPAT4-2编码379个氨基酸,所得蛋白质的相对分子质量和等电点分别为43.16 kDa和8.93。他们均具有LPLAT_LCLAT1样结构域,同属于LPLAT超基因家族。比较所用油菜序列SNP分析显示,LPAT4共存在41个多态性位点,其中单变异位点7个,简约信息位点...

To further study on LPAT4 of Brassica napus, a pair of primers were designed to obtain the full length CDs sequence of rapeseed LPAT4 gene,according to the homologous comparison of B. campestris,B. oleracea and assembled EST sequence,which was strong similarity to the LPAT4 gene of Arabidopsis thaliana by searching bioinformatics at Geboc database. In this paper,two copies of full length CDs sequences(1 143,1 140 bp)have been cloned,these clones were designated as BnLPAT4-1 and BnLPAT4-2,respectively. BnLPAT4-1 encoded a protein of 380 amino acids with the molecular weights of 43 . 29 kDa and ...