期刊论文

Study on Chlorophyll Content and Expression of Fatty Acid Synthesis Related Genes in Rape under Different Fertilizer and Density Conditions

中文

华北农学报

1000-7091

2018

33

1

127-134

10.7668/hbnxb.2018.01.020

湖南省省长项目（湘财农指[2016]114号）； 作物种质创新与资源利用国家重点实验室培育基地开放课题（15KFXM16）；

本校为第一机构

提高油菜籽品质一直是育种家们不懈追求的目标之一,不同的栽培措施对油菜不通脂肪酸合成有一定影响,为从分子方面促进油菜脂肪酸组成改良研究的发展,以甘蓝型油菜420为材料,采用三元二次回归正交组合设计方法,对不同生育时期、不同栽培条件下叶绿素含量及fad2、fad3基因的表达量变化情况进行分析。研究发现:叶绿素含量随着氮、硼施用量的增加呈先升后降,随种植密度增加而降低; fad2、fad3的表达量变化与叶绿素含量规律相同,且通过线性拟合分析发现,fad2、fad3基因表达量与叶绿素含量呈正相关关系。结果表明,fad2、fad3基因在A2B1C2与A2B1C3处理下均有较高表达量,而在A1B3C1、A3B3C1及A1B3C3处理...

The fatty acid composition is one of the important goals to improve rapeseed quality,different envi-ronment and cultivation conditions can altered fatty acid composition.This experiment was to promote the improve-ment of rapeseed fatty acid composition from molecular aspects. Brassica napus 420 was used as material, the de-sign method of ternary quadratic regression orthogonal combination was adopted, chlorophyll content and fad2 and fad3 gene were studied in different periods and different cultivation conditions. And found that:the content of chlo-rophyll increased first and then decreased wi...