期刊论文

Functional Analysis of A7- FT Promoter in Brassica napus L

中文

华北农学报

1000-7091

2019

34

2

59-65

10.7668/hbnxb.201751081

(2015CB150206):国家"973"计划项目 :湘财农指(2016)114 号

本校为第一机构

为了利用PCR技术得到甘蓝型油菜A7-FT基因启动子序列,根据甘蓝型油菜全基因组序列,利用启动子在线预测软件预测其功能与结构,根据其预测的顺式元件的分布,从5'端开始缺失的方式获得5个不同片段长度的启动子序列。构建含不同片段长度启动子的GUS基因表达载体,利用农杆菌介导拟南芥,得到T2幼苗,经过GUS染色与脱色,探讨A7-FT基因启动子的功能,为研究甘蓝型油菜开花调控机制提供理论基础。通过PCR技术从甘蓝型油菜湘油15号基因DNA中获得A7-FT基因启动子序列。利用PLACE和PlantCARE在线工具对该段序列进行预测,发现A7-FT基因启动子除了存在启动子核心元件CAATbox和TATAbox,还有光应答元件、激素应答元...

Based on the whole genome sequence of Brassica napus,the sequence of A7-FT gene promoter was obtained by PCR. The promoter online prediction software was used to predict the function and structure of the promotor, and five promoter sequences with different fragment lengths were obtained from the 5'-end deletion according to the distribution of predicted cis-elements. The GUS gene expression vector containing promoters with different fragment lengths was constructed and T2 seedlings were obtained by Agrobacterium-mediated Arabidopsis thaliana. After GUS staining and decolorization,the function ...