期刊论文

Amino Acid Sequence Analysis of Mycoplasma hyopneumoniae P36,P46,P97 R1 and P146 in Slaughter Pigs

中文

华北农学报

1000-7091

2019

34

2

212-220

10.7668/hbnxb.201751207

国家重点研发项目（2017YFD0500102）；

本校为第一机构

为了解猪肺炎支原体流行菌株遗传变异情况,选取2014- 2017年不同时间、地点收集的17头猪肺炎支原体阳性屠宰猪肺脏灌洗液DNA为模板,进行猪肺炎支原体P36(编码具有强免疫原性的胞浆蛋白)、P46(编码具有强免疫原性的表面蛋白)、P97 R1区、P146高变区(猪肺炎支原体黏附气管纤毛细胞的重要功能区域)基因的PCR扩增、测序。结合GenBank登录的猪肺炎支原体相关序列进行氨基酸序列的比对分析。结果表明,比对的序列之间, P36、P46蛋白氨基酸序列同源性均在98.9%以上,但P97功能区R1区、P146多变区在菌株间存在较大差异。11个屠宰猪样本和5个GenBank登录菌株P97 R1区重复氨基酸序列数量达到14个以上,而GenB...

To understand the genetic variation of Mycoplasma hyopneumoniae (M. hyo) epidemic strain,a conventional PCR was employed to amplify the fragments of M. hyo P36,P46,P97 R1 and P146 gene from the DNA mix of bronchoalveolar lavage fluid of 17 slaugher pigs,which had been tested positive to M. hyo,and the PCR products were sequenced directly and analyzed. The deduced amino acid sequences of P36 and P46 were conservative between M. hyo of 17 slagughter pig lung samples and those strains registered in GenBank,with a homology of more than 98.9%. Among the amino acid sequences,there were large variati...