期刊论文

Studies on the Expression of Phosphodiacylglycerol Acyltransferase (BnPDAT1) in Brassica napus

中文

华北农学报

1000-7091

2019

34

1

12-18

10.7668/hbnxb.201751114

31401419:国家自然科学基金 13B051:湖南省教育厅优秀青年项目

本校为第一机构

为探究甘蓝型油菜中BnaPDAT1基因表达特性与油脂合成之间的关系。选用2个含油量具有显著差异的甘蓝型油菜双低品系855（49. 72%）和868（35. 06%）,以qRT-PCR方法检测BnaPDAT1各拷贝在两品系油菜中的表达规律,同时以薄层层析（TLC）和气相色谱（GC）检测两品系油菜中TAG的积累规律。结果表明:授粉后种子中BnaPDAT1基因及其3个拷贝表达量均呈先升高后降低的趋势,花、叶片中均有BnaPDAT1表达,三拷贝表达存在差异,但表现整体调控的特点。两品系油菜叶（Bna A10. PDAT1除外）和授粉后20 d的种子中BnaPDAT1及其3个拷贝表达量具有极显著差异,其余各时期两品系间表达差异规律不明显,BnaPDAT1在高含油量...

This paper aims to investigate the relationship between the expression characteristics of BnaPDAT1 gene and oil synthesis in Brassica napus L.. Two Brassica napus L. varieties,double-low strains 855 (49.72%) and 868 (35.06%),were selected as materials,from which 3 copies of BnaPDAT1 were detected by qRT-PCR. The regularity was expressed,and the accumulation of TAG in the two strains of rapeseed was detected by thin layer chromatography (TLC) and gas chromatography (GC). The results showed that the expression of BnaPDAT1 gene and its three copies in seeds increased first and then decreased afte...