期刊论文

Cloning and Prokaryotic Expression of Transcription Factor StWRKY57 Gene in Potato

中文

华北农学报

1000-7091

2021

36

01

10-17

10.7668/hbnxb.20191222

国家自然科学基金项目（31570372）； 江西省自然科学基金项目（20192BAB204012）； 江西省教育厅科技项目（GJJ180843）；

本校为其他机构

为了研究马铃薯WRKY57基因（PGSC transcript number PGSC0003DMT400072958）功能,通过RT-PCR克隆得到马铃薯耐旱品种PB06的StWRKY57基因cDNA序列,命名为StWRKY57-PB。利用生物信息学方法对其序列特征进行了分析;以pGEX-KG为基本骨架,构建了StWRKY57-PB蛋白的原核表达载体并在大肠杆菌中进行了表达和纯化分析。结果表明,StWRKY57-PB基因开放阅读框（ORF）长981 bp,编码蛋白属于WRKY转录因子家族Ⅱc类,含326个氨基酸残基,含有WRKY结构域（WRKYGQK）和一个类锌指结构基序(C-X4-C-X23-H-X1-H)。序列比对及构建系统发育树结果表明,它与马铃薯基因组数据库及NCBI公布的WRKY57（XP006348711.1）有4个氨...

In order to explore the function of StWRKY57 (PGSC0003DMT400072958) in potato,a full-length cDNA sequence of the homologous StWRKY57 gene in Solanum tuberosum PB06 named StWRKY57-PB was cloned by RT-PCR and bioinformatics methods were used to analyze the sequence characteristic of StWRKY57-PB.For prokaryotic expression and purify,the prokaryotic expression vector of StWRKY57-PB was constructed based on pGEXKG,then fusion protein was induced in Escherichia coli strains.The results showed that the StWRKY57-PB gene contained a 981 bp open reading ...